| 品牌: 美国 NewEast Biosciences |
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| 货号: 26905 |
| 抗原: Full Recombinant Active Cdc42 |
| 规格: 30 微升 |
| 产品全名: Anti-Cdc42-GTP Monoclonal Antibody |
| 应用: IP, IHC, IF |
| 种属反应性的选择: Human, Mouse and Rat |
| 物种/宿主: Mouse |
| 货号: 26905 |
| 规格: 30 µL |
| 基因符号: CDC42 |
| 描述: Anti-Cdc42-GTP Monoclonal Antibody |
| 背景介绍: Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| 免疫原: Recombinant full length protein of active Cdc42 |
| 经过测试的应用: IP, IHC |
| 推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG1 |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-Cdc42-GTP monoclonal antibody recognizes active Cdc42 from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
IP-蛋白质印迹: IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Cdc42-GTP mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti-Cdc42 mouse monoclonal antibody (Cat. # 26008). |
免疫荧光: |
