货号: 26907 |
规格: 30 µL |
基因符号: Gnao |
描述: Anti Gαo-GTP Mouse Monoclonal Antibody |
背景介绍: Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored. |
免疫原: Recombinant full length protein of active Gαo |
经过测试的应用: IP, IHC, IF |
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
浓度: 1 mg/ml |
宿主: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG1 |
纯度: Purified from ascites |
防腐剂: no |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Anti-active Gαo antibody recognizes active Gαo of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹: Gαo Activation Assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti-Gαopolyclonal antibody (Cat. No. 21015). |
Gαo-GTP 小鼠单抗